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Marcia Maria de Oliveira  Buanafina

Marcia Maria de Oliveira Buanafina

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Senior Research Scientist

319 Mueller
University Park, PA 16802
Phone: (814)867-0223
Lab Address: 319 Mueller Lab
Email:

Education

  1. B.A. in Agronomic Engineering ,Universidade Federal Rural de Pernambuco, Brazil
  2. PhD. in Plant Breeding and Genetics, University of Wales/IBERS, Aberystwyth, Wales/ UK

Postdoc Training

  1. IGER (Institute of Grassland and Environmental Research), Plas Gogerddan, Aberystwyth University, Wales, UK.

Honors and Awards

  1. CNPq Graduate Research Fellow
  2. IGER/GCI Post Doctoral Research Associate

Research Interests

Feruloylation, lignification and degradability of grass cell walls

Grass cell walls are characterized by a large quantity of esterified ferulates. Feruloylation of AX is important because it directly cross-links xylans and because ferulate esters become incorporated into lignin generating a  ferulate-polysaccharide-lignin complex. The accumulation of cell wall ferulates, the cross-linking of xylan appears to play a role in different plant processes.

Buanafina lab is interested in understanding the role of feruloylation in grass cell walls, identifying gene(s) involved, understanding lignin deposition and arabinoxylans biosynthesis. It is also part of our research to study the expression of  fungal  cell wall degrading enzymes in planta in order to test if their  expression can assist the digestion of polysaccharides and improve the nutritional properties of grass as a ruminant feed or as a bioenergy crop. We make use of forward and reverse genetic approaches, spectrophotometric, chromatography and microscopy techniques, chemical mutagenesis and genetic transformation.

 

Selected Publications

  • Michael L. Robbins, Ansuman Roy, Po-Hao Wang, Iffa Gaffoor, Rajandeep S. Sekhon, Marcia M. de O. Buanafina, Jai S. Rohila, Surinder Chopra (2013). Comparative Proteomics Analysis by DIGE and  iTRAQ Provides Insight into the Regulation of  Phenylpropanoids in Maize. Journal of Proteomics. 93:254-275
  • Buanafina, M.M de O., Langdon T., Dalton, S. and  Morris, P (2012). Expression of a Trichoderma reesei ß-1,4 endo-xylanase in tall fescue modifies cell wall structure and digestibility and elicits pathogen defence responses”. Planta 236: 1757-1774
  • Buanafina, M M de O and Fescemyer, H. W. (2012)  Modification of esterified cell wall phenolics increases vulnerability of tall fescue to insect herbivory. Planta  236: 513-523
  • Buanafina, M.M de O., Langdon T., Hauck B., Dalton, S. Timms-Tavarella, E. and  Morris, P. (2010). Targeting expression of a fungal ferulic acid esterase to the apoplast, endoplasmic reticulum or Golgi can disrupt feruloylation of the growing cell wall and increase the biodegradability of tall fescue (Festuca arundinacea). Plant Biotech. J. 8:316-331.
  • Buanafina, M.M de O. (2009). Feruloylation in Grasses: Current and Future Perspectives. Molecular Plant.2:861-872.
  • Buanafina, M.M de O. (2008). Engineering Grasses with Reduced Cross-linking of Cell Walls:Impact for Animal and Biofuel Industries. Information Systems for Biotechnology.  August, 8-11.
  • Buanafina, M.M de O., Langdon T., Dalton S.J, Hauck B., Morris P. (2008). Expression of a fungal ferulic acid esterase increases cell wall digestibility of tall fescue (Festuca arundinacea). Plant Biotechnology J6: 264-280.
  • Buanafina, M.M.de O.,Langdon, T., Hauck, B., Dalton, S.J.,  and Morris, P. (2006). Manipulating the phenolic acid content and digestibility of Italian ryegrass (Lolium multiflorum) by vacuolar targeted expression of a fungal ferulic acid esterase.Appl.Bioch.Biotech. 129-132: 416-426.
  • Buanafina, M. M. de O. , Morris, P. , Langdon, T. , Dalton, S. J. , Hauck, B., Porter,H. (2005) Manipulating the phenolic acid content and digestibility of forage grasses by targeted expression of fungal cell wall degrading enzymes. Molecular Breeding for the Genetic Improvement of Forage Crops and Turf. Proceedings of the 4th International Symposium on the Molecular Breeding of Forage and Turf. XXth International Grassland Congress, July 2005, Aberystwyth, Wales Humphreys, M. O., eds. p239  Wageningen Academic Publishers.
  • Dalton, S. J. , Robson, P. R. H. , Buanafina, M. M. de O.Bettany, A. J. E., Timms, E. J., Morris, P. (2005) A comparison of hygromycin and paromomycin selection strategies in the genetic transformation of seven Lolium, Festuca, Poa and Agrostis species Molecular Breeding for the Genetic Improvement of Forage Crops and Turf. Proceedings of the 4th International Symposium on the Molecular Breeding of Forage and Turf. XXth International Grassland Congress, July 2005, Aberystwyth, Wales Humphreys, M. O., eds. p237 Wageningen Academic Publishers.
  • Dalton, S. J. , Robson, P. R. H. , Buanafina, M. M. de O. , Bettany, A. J. E., Timms, E.,Wiffen, D. (2005) Nylon mesh as an improved support for bombarded calli orcellsuspensionsMolecular Breeding for the Genetic Improvement of Forage Crops and Turf. Proceedings of the 4th International Symposium on the Molecular Breeding of Forage and Turf. XXth International Grassland Congress, July 2005, Aberystwyth, Wales   Humphreys, M. O., eds.   p236  Wageningen Academic Publish.
  • Buanafina, M.M de O., Langdon T., Hicks, H.C., Hauck B., Dalton S.J. and Morris P. (2002).Targeted expression of a ferulic acid esterase from Aspergillus niger in leaves of forage grasses. In:Durand J-L, Emile J-C, Huyghe C. and Lemaire G. (eds). Multi-Function Grasslands: Quality forages, Animal Products and Landscapes. Proc. 19th European Grassland Federation, La Rochelle (France) 27-30 May 2002. Pg. 66-67.